EFFECT OF PUGUNTANO LEAF EXTRACT (CURANGAFEL-TERRAE MERR) ON INTERLEUKIN-1 EXPRESSION α SION OF CHOLESTEATOMA MATRIX CULTURES WITH ATTICOANTRAL CHRONIC SUPURATIVE OTITIS MEDIA

Elvien Dwi Saleh, Harry Agustaf Asroel, Adlin Adnan, Restu Syamsul Hadi

Abstract


Chronic otitis media (COM) with or without cholesteatoma is a major global health problem, especially in
developing countries. Until today, there is no other treatment for COM with cholesteatoma (COMch) other
than surgery. Puguntano (Curanganfel-terrae Merr) is a plant from Scrophulariaceae family. It can be found in Asia, especially
in China, India, Philippines, Myanmar, Malaysia and Indonesia. This plant has traditionally used as stimulant, antidiuretic,
treatment for malaria, hepatoprotective drug, antipyretic, antiherpetic drug, anti-cancer, antihyperglycemic, antioxidant and
anti-inammatory. The objective of this study is to evaluate the effect of ethanol extract from Puguntano leaves on Interleukin-1α
expression level in cholesteatoma matrix cultures with atticoantral type chronic supurative otitis media. This study is an in vitro
experimental research comparing two groups, a keratinocyte cholesteatoma culture with and without Puguntano extract. A
group without extract Puguntano was divided into 4 different groups: group with Puguntano extract 1 mg/ml, 2 mg/ml, 4 mg/ml
and control group.Interleukin-1α level in cholesteatoma culture is inversely proportional to Puguntano extract. Interleukin-1α
expression level in group with Puguntano extract 1 mg/ml is 4,335 pg/ml; 2 mg/ml is 3,83 pg/ml; and 4 mg/ml is 2,74 pg/ml. Those
results showed the decreasing level of Interleukin-1α expression compared to control group, which is 8,2889 pg/ml.


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