A STUDY ON IDENTIFICATION AND SPECIATION OF MEDICALLY IMPORTANT CANDIDA SPECIES ISOLATED FROM VARIOUS CLINICAL SAMPLES BY USING HICROME CANDIDA.

Mr Jogender, Dr Anjali Kulshrestha, Dr Suman Rishi

Abstract


Candida albicans remains the most frequently isolated species, but an increase in the prevalence of Non-albicans candida is a matter of concern for
various laboratories, as NAC show less susceptibility to antifungal agents particularly azoles1-3. Routinely used conventional methods are
cumbersome and time consuming. Hence the present study aimed at species identication of candida isolated from various clinical specimens and
also to evaluate the usefulness of HiCrome Candida differential agar as compared to routine conventional method for speciation of candida. A total
of 51 non repetitive clinical isolates of Candida were obtained from various clinical samples. Candida albicans was the major species accounting
for 23 (45.10%) of the total isolates. Non albicans Candida constituted 17 (33.33%) of C. tropicalis, 9 (17.64%) of C. krusei and 2 (3.92%) of C.
parasilosis. 49 Candida species which were correctly identied by HiCrome Candida agar except 2 species of C.parasilosis (identied by
conventional method) which were identied as C. glabrata by Hicrome Candida agar.


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