COMPARATIVE EVALUATION OF THE ANTIBACTERIAL EFFICACY OF CALCIUM HYDROXIDE AS AN INTRACANAL MEDICAMENT AND COMBINATION OF CALCIUM HYDROXIDE WITH FOOD PRESERVATIVES AND PROTON PUMP INHIBITOR AGAINST ENTEROCOCCUS FAECALIS-AN INVITRO STUDY

Dr Suman Kar, Dr. Swarupananda Bera, Dr. Debjyoti Karmakar

Abstract


AIM: To evaluate the antimicrobial effcacy of nisin and calcium hydroxide with and without omeprazole against
Enterococcus faecalis.
MATERIALS AND METHODS: The antibacterial effect of the following experimental groups as intracanal
medicaments(Group I— Ca(OH)2, Group II – Ca(OH)2 + Nisin, Group III - Ca(OH)2 + Nisin + PPI, Group IV— Ca(OH)2 +
PPI) was evaluated using the agar diffusion test for a time period of 24 hours. The minimum inhibitory concentration
(MIC) against E.faecalis were also determined. Statistical analysis was performed using Kruskal–Wallis Test and chisquare
test.
RESULTS: In this study the antibacterial efficacy is detected by the formation of the zone of inhibition around the wells
inoculated with the experimental groups. Groups I, II, III and IV showed inhibitory zones. In group IV the maximum
diameter of 22 mm is obtained . The MIC values for the experimental groups I, II, III, and IV were
0.45%,0.2mg/ml,0.45%+0.03mg/ml, ≤0.01% respectively.
CONCLUSION: The antimicrobial efficacy of omeprazole (PPI) combined with calcium hydroxide shows the maximum
zone of inhibition and according to this study; this combination shows the most potent intracanal medicaments.


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References


Love RM.Enterococcus faecalis—a mechanism for its role in endodontic failure. Int Endod J 2001 Jul; 34(5):399-405.

Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis—its role in root canal treatment failure and current concepts in retreatment. J Endod 2006 Feb; 32(2):93-98.

Sirén EK, Haapasalo MP, Waltimo TM, Ørstavik D. In vitro antibacterial effect of calcium hydroxide combined with chlorhexidine or iodine potassium iodide on Enterococcus

faecalis. Eur J Oral Sci 2004 Aug; 112(4):326-331.

Tong Z, Ling J, Lin Z, Li X, Mu Y. The effect of MTADN on 10 Enterococcus faecalis isolates and bioflm: an in vitro study.J Endod 2013 May; 39(5):674-678.

Hemadri M, Thakur S, Sajjan G. Nisin vs. calcium hydroxide—antimicrobial effcacy on Enterococcus faecalis—an in-vitro study. Int J Contemp Dent 2011 Jun;2(3):55-61.

Chinni SK, Veni AB, Srinivasan MR, Rajamani I. An in vitro investigation of a newer intracanal medicament Nisin on Enterococcus faecalis in comparison with chlorhexidine and

calcium hydroxide. J Int Clin Dent Res Organ 2011 Jan; 3(1): 21-24.

Severina E, Severin A, Tomasz A. Antibacterial effcacy of nisin against multidrug-resistant Gram-positive pathogens.J Antimicrob Chemother 1998 Mar; 41(3):341-347.

Tong Z, Zhou L, Li J, Jiang W, Ma L, Ni L. In vitro evaluation of the antibacterial activities of MTAD in combination with nisin against Enterococcus faecalis. J Endod 2011

Aug;37(8):1116-1120

SHANDS. Drugs and therapy bulletin. Vol. 15(1). Gainesville (FL): Shands at the University of Florida; 2001.

Wagner C, Barth VC Jr, de Oliveira SD, Campos MM. Effectiveness of the proton pump inhibitor omeprazole associated with calcium hydroxide as intracanal medication: an in vivo

study. J Endod 2011 Sep; 37(9):1253-1257.

Jack FW, Tagg JR, and Ray B. Bacteriocins of gram positive bacteria. Microbiol Rev 1995 Jun; 59(2):171-200.

Du Plessis DJ, Nouwen N, Driessen AJ. The Sec translocase. Biochem Biophys Acta 2011 Mar; 1808(3):851-865.

Crandal AD, Montville T. Nisin resistance in Listeria monocytogenes ATCC 700302 is a complex phenotype. Appl Environ Microbiol 1998 Jan;64(1):231-237


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