MUSHROOM POISONING: IDENTIFICATION AND QUANTIFICATION OF TOXINS WILL TOXINS IDENTIFICATION HELP IN PREVENTION OF ACCIDENTAL POISONING?

D. Slong, J B Wahlang, Rangme B. Y. Marbaniang, Arky Jane Langstieh, D. Ropmay, I. Tiewsoh, J A Lyngdoh

Abstract


Amatoxins commonly found in Amanita phalloides is the main constituents of toxins present in most toxic mushroom
specimens containing α-amanitin, β-amanitin, γ-amanitin, ε-amanitin, amanin, amaninamide, amanullin, amanullinic acid,
and proamanullin. RP-HPLC analysis of toxins Chromatography: The method of Ismail Yilmaz et. al, is followed using C18
(Agilent Technologies) at UV detection 303nm for amatoxins and 291nm for phallotoxins. The mobile phase in isocratic
pump with a flow rate of 1ml/min consisting of 0.05M ammonium acetate (pH 5.5 with acetic acid) and acetonitrile
(90:10v/v). The geographical variations determine the content of the toxins and it brings a landmark to create awareness
to the community for such mushroom specimens. We hope that analyzing the toxin content in the coming years will be of
great service to the Physician and the community as well.


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